Comparison of molecular and conventional methods for typing of enteroviral isolates.

نویسندگان

  • Darly Joseph Manayani
  • R V Shaji
  • G John Fletcher
  • Thomas Cherian
  • N Murali
  • N Sathish
  • Tilak Solomon
  • Chandran Gnanamuthu
  • Gopalan Sridharan
چکیده

Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 40 3  شماره 

صفحات  -

تاریخ انتشار 2002